Title: Principal Investigator E-mail: zhouxi@wh.iov.cn


2001, B.S., College of Life Sciences, Wuhan University, Wuhan, China

2008, Ph.D., the University of Texas MD Anderson Cancer Center

Research Interests

RNA viruses (flavivirus and enterovirus)

Virus interaction with antiviral immunity


1. Shu T, Ning W, Wu D, Xu J, Han Q, Huang M, Zou X, Yang Q, Yuan Y, Bie Y, Pan S, Mu J, Han Y, Yang X, Zhou H, Li R, Ren Y, Chen X, Yao S, Qiu Y*, Zhang DY*, Xue Y*, Shang Y*, Zhou X*. (2020) Plasma Proteomics Identify Biomarkers and Pathogenesis of COVID-19.Immunity. 53(5):1108-1122.e5.

2. Wang C, Xu J, Wang S, Pan S, Zhang J, HanY, Huang M, Wu Di, Yang Q, Yang X, Yang Y, Shu T, Zou X, Li R, Luo Y, Yao R, Wang Y, Qiu Y, Wang Y, Zhang D-Y, Yao Q*, Yan Y*, Zhou X* and Shang Y*(2020) Imaging Mass Cytometric Analysis of Postmortem Tissues Reveals Dysregulated Immune Cell and Cytokine Responses in Multiple Organs of COVID-19 Patients. Frontiers in Microbiology. doi:10.3389/fmicb.2020.600989

3. Zhao Y, Shang Y, Ren Y, Bie Y, Qiu Y, Yuan Y, Zhao Y, Zou L, Lin S-H*, Zhou X*.(2020) Omics study reveals abnormal alterations of breastmilk proteins and metabolites in puerperant women with COVID-19. Signal Transduction &Targeted Therapy. 2020; 23, 5(1):247

4. Mu J, Fang Y, Yang Qi, Shu T, Wang A, Huang M, Jin L, Deng F, Qiu Y*, Zhou X*. (2020) SARS-CoV-2 N protein antagonizes type I interferon signaling by suppressing phosphorylation and nuclear translocation of STAT1 and STAT2. Cell Discovery. 2020, 15;6:65

5. Han Y, Yang Q, Liu Y, Shu T, Yue L, Xiao T, Zeng Q, Wu Y, Zhou X*, Zhang DY*. (2020) Feasibility Study of Mixing Throat Swab Samples for Severe Acute Respiratory Syndrome Coronavirus-2 Screening. Virologica Sinica, 8;1-3. 

6. Wu D, Shu T, Yang X, Song J-X, Zhang M, Yao C, Liu W, Huang M, Yu Y, Yang Q, Zhu T, Xu J, Mu J, Wang Y, Wang H, Tang T, Ren Y, Wu Y, Lin S-H*, Qiu Y*, Zhang D-Y*, Shang Y*,Zhou X*.(2020) Plasma metabolomic and lipidomic alterations associated with COVID-19.National Science Review, 7: 11571168

7. Shu T, Huang M, Wu D, Ren Y, Zhang X, Han Y, Mu J, Wang R, Qiu Y*, Zhang D-Y*,Zhou X*. (2020) SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts. Virologica Sinica. 35(3):321-329.

8.  Ren Y, Shu T, Wu D, Mu J, Wang C, Huang M, Han Y, Zhang X, Zhou W, Qiu Y, Zhou X*.(2020) The ORF3a protein of SARS-CoV-2 induces apoptosis in cells. Cellular & Molecular Immunology,2020 Aug;17(8):881-883.

9. Mu J, Zhang H, Li T, Shu T, Qiu Y, Zhou X*. (2020) The 3A protein of coxsackievirus B3 acts as a viral suppressor of RNA interference. J Gen Virol, 101(10):1069-1078.

10. Mu J, Xu J, Zhang L, Shu T, Wu D, Huang M, Ren Y, Li X, Geng Q, Xu Y, Qiu Y*, Zhou X*. (2020) SARS-CoV-2-encoded Nucleocapsid Protein Acts as a Viral Suppressor of RNA interference in Cells. Science China Life Sciences, 2020 Sep;63(9):1-4

11. Qiu Y, Xu Y-P, Wang M, Miao M, Zhou H, Xu J, Kong J, Zheng D, Li R-T, Zhang R-R, Guo Y, Li X-F, Cui J, Qin C-F*, Zhou X*. (2020) Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes. Science Advances 6: eaax7989.

12. Qian Q, Zhou H, Shu T, Mu J, Fang Y, Xu J, Li T, Kong J, Qiu Y*, Zhou X*. (2020) The Capsid Protein of Semliki Forest Virus Antagonizes RNA Interference in Mammalian Cells. J Virol. 94(3): e01233-19 (J Virol Spotlight Article)

13. XuY-P, Qiu Y, Zhang B, Chen G, Chen Q, Wang M, Mo F, Xu J, Wu J, Zhang R-R, Cheng M-L, Zhang N-N, Lyu B, Zhu W-L, Wu M-H, Ye Q, Zhang D, Man J-H, Li X-F, Cui J, Xu Z, Hu B*, Zhou X*, Qin C-F*. (2019) Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids. Cell Res.29: 265-273. (Cover story)

14. Shu T, Gan T, Bai P, Wang X, Qian Q, Zhou H, Cheng Q, Qiu Y, Yin L, Zhong J, Zhou X*. (2019) Ebola Virus VP35 Has Novel NTPase and Helicase-like Activities. Nucleic Acids Res.47(11): 5837–5851.

15. Zhou H, Qian Q, Shu T, Xu J, Kong J, Mu J, Qiu Y*, Zhou X*. Hepatitis C Virus NS2 Protein Suppresses RNA Interference in Cells. Virol. Sin. DOI: 10.1007/s12250-019-00182-5.

16. Miao M, Yu F, Wang D, Tong Y, Yang L, Xu J, Qiu Y, Zhou X*, Zhao X*. (2019) Proteomics Profiling of Host Cell Response via Protein Expression and Phosphorylation upon Dengue Virus Infection. Virol. Sin. 34: 549–562.

17. Liu YX, Wang M, Liu X, Quan J, Fang Y, Liu YJ, Qiu Y, Yu Y, Zhou X*. (2019) Drosophila Trf4-1 involves in mRNA and primary miRNA transcription. Biochem Biophys Res Comm.511(4): 806-812

18. Liu YJ, Fang Y, Liu YX, Wang Z, Lyu B, Hu Y,Zhou X*. (2019) Opposite effects of Drosophila C3PO on genesilencing mediated by esi-2.1 and miRNAbantam. Acta Biochim Biophys Sin, 51(2), 131–138.

19. Qiu Y and Zhou X*. (2018) STING: From Mammals to Insects. Cell Host Microbe24(1): 5-7.

20. Li T-F, Hosmillo M, Schwanke H, Shu T, Wang Z, Yin L, Curry S, Goodfellow IG and Zhou X*. (2018) Human Norovirus NS3 has RNA Helicase and Chaperoning Activities. J Virol. 92(5): e01606-17

21. Wang Z, Xia X, Yang X, Zhang X, Liu Y, Wu D, Fang Y, Liu Y, Qiu Y and Zhou X*. (2017) A Picorna-like Virus Suppresses the N-end Rule Pathway to Inhibit Apoptosis. eLife 6: e30590, DOI: 10.7554/eLife.30590

22. Qiu Y, Xu Y, Zhang Y, Zhou H, Deng Y-Q, Li X-F, Miao M, Zhang Q, Zhong B, Hu Y, Zhang F-C, Wu L, Qin C-F* and Zhou X*. (2017) Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals. Immunity 46(6): 992–1004.

23. Yang J, Qian Q, Li T-F, Yang X, Won S J, Zhou X*. (2017) Cypovirus capsid protein VP5 has nucleoside triphosphatase activity. Virol Sin. 32 (4): 328–330.

24. Wang Z, Wu D, Liu Y, Xia X, Gong W, Qiu Y, Yang J, Zheng Y, Li J, Wang YF, Xiang Y, Hu Y, Zhou X*. (2015) Drosophila Dicer-2 has an RNA interference-independent Function that Modulates Toll Immune Signaling. Science Advances 1: e1500228.

25. Xia H, Wang P, Wang GC, Yang J, Sun X, Wu W, Qiu Y, Shu T, Zhao X, Yin L, Qin CF, Hu Y, Zhou X*. (2015) Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-independent RNA Chaperone. PLoS Pathogens 11(7): e1005067.

26.  Yang J, Xia H, Qian Q, Zhou X*(2015) RNA chaperones encoded by RNA viruses, Virol Sin. 30(6): 401-409.

27. Cui L, Wang H, Ji Y, Yang J, Xu S, Huang X, Wang Z, Qin L, Tien P, Zhou X, Guo D*, Chen Y*. (2015) The Nucleocapsid Protein of Coronaviruses Acts as a Viral Suppressor of RNA Silencing in Mammalian Cells. J Virol. 89(17): 9029-9043.

28. Yang J, Cheng Z, Zhang S, Xiong W, Xia H, Qiu Y, Wang Z, Wu F, Qin CF, Yin L, Hu Y and Zhou X* (2014) A Cypovirus VP5 Displays the RNA Chaperone-like Activity that Destabilizes RNA Helices and Accelerates Strand Annealing. Nucleic Acids Res. 42(4): 2538-2554.

29. Qiu Y, Miao M, Wang Z, Liu Y, Yang J, Xia H, Li XF, Qin CF, Hu Y and Zhou X* (2014) The RNA Binding of Protein A from Wuhan Nodavirus is Mediated by Mitochondrial Membrane Lipids. Virology 462-463: 1-13.

30. Tang F, Xia H, Wang P, Yang J, Zhao T, Zhang Q, Hu Y* and Zhou X*. (2014) The Identification and Characterization of Nucleic Acid Chaperone Activity of Human Enterovirus 71 nonstructural protein 3AB. Virology 464-465: 353-364.

31. Qiu Y, Wang Z, Liu Y, Han Y, Miao M, Qi N, Yang J, Xia H, Li X, Qin CF, Hu Y* and Zhou X* (2014) The Self-interaction of a Nodavirus Replicase is Enhanced by Mitochondrial Membrane. PLoS ONE 9(2): e89628.

32. Wu W, Wang Z, Xia H, Liu Y, Qiu Y, Liu Y, Hu Y and Zhou X* (2014) Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity. PLoS ONE 9(1): e86876.

33. Zhao H, Jiang T, Zhou X-Z, Deng YQ, Li XF, Chen SP, Zhu SY, Zhou X, Qin ED and Qin CF*. (2014) Induction of Neutralizing Antibodies against Four Serotypes of Dengue Viruses by MixBiEDIII,aTetravalent Dengue Vaccine. PLoS ONE 9(1): e86573.

34. Wang Z, Qiu Y, Liu Y, Qi N, Si J, Xia X, Wu D, Hu Y and Zhou X* (2013) Characterization of a Nodavirus Replicase Revealed a De Novo Initiation Mechanism of RNA Synthesis and Terminal Nucleotidyl Transferase Activity. J Biol. Chem.288(43): 30785-801.

35. Cheng Z, Yang J, Xia H, Qiu Y, Wang Z, Han Y, Xia X, Qin CF, Hu Y* and Zhou X* (2013) The Nonstructural Protein 2C of a Picorna-like Virus Displays Nucleic Acid Helix Destabilizing Activity that can be Functionally Separated from its ATPase Activity. J Virol.87: 5205-18 (MDLinx Featured Article)

36. Qiu Y, Wang Z, Qi N, Si J, Liu Y, Xiang X, Hu Y and Zhou X*. (2013) Membrane association of Wuhan Nodavirus protein A is required for its ability to accumulate genomic RNA1 template. Virology 439: 140-151 

37. Han Y, Wang Q, Qiu Y, Wu W, He H, Zhang J, Hu Y* and Zhou X* (2013) Periplaneta fuliginosa Densovirus Nonstructural Protein NS1 contains an endonuclease activity that is regulated by its phosphorylation. Virology 437: 1-11.

38. Qiu Y*, Wang Z, Liu Y, Qi N, Si J, Xiang X, Xia X, Hu Y and Zhou X(2013) Newly discovered insect RNA viruses in China. Sci China Life Sci. 56(8):711-714

39. Liu ZY, Li XF, Jiang T, Deng YQ, Zhao H, Wang HJ, Ye Q, Zhu SY, Qiu Y, Zhou X, Qin ED, Qin CF (2013) Novel cis-acting element within the capsid-coding region enhances flavivirus viral-RNA replication by regulating genome cyclization. J Virol. 87:6804-18.

40. Qi N, Zhang L, Qiu Y, Wang Z, Si J, Liu Y, Xiang X, Xie J, Qin CF, Zhou X*, HuY (2012) Targeting of Dicer-2 and RNA by a Viral RNA Silencing Suppressor in Drosophila Cells. J Virol. 86:5763-5773. (J Virol Spotlight Article)

41. Wang Q, Han Y, Qiu Y, Zhang S, Tang F, Wang Y, Zhang J, Hu Y* and Zhou X* (2012) Identification and Characterization of RNA Duplex Unwinding and ATPase Activities of an Alphatetravirus Superfamily 1 Helicase. Virology 433: 440-448.

42. Ye S., Xia H, Dong C, Cheng Z, Xia X, Zhang J, Zhou X*, and Hu Y* (2012) Identification and Characterization of Iflavirus 3C-like Protease Processing Activities. Virology 428: 136-145.

43. Ye Q, Li XF, Zhao H, Li SH, Deng YQ, Cao RY, Song KY, Wang HJ, Hua RH, Yu YX, Zhou X, Qin ED, Qin CF. (2012) A single nucleotide mutation in NS2A of Japanese encephalitis-live vaccine virus (SA14-14-2) ablates NS1' formation and contributes to attenuation. J Gen Virol. 93:1959-64.

44. Qi N, Cai D, Qiu Y, Xie J, Wang Z, Si J, Zhang J, Zhou X* and Hu Y* (2011) RNA Binding by a Novel Helical Fold of B2 Protein from Wuhan Nodavirus Mediates RNAi Suppression and Promotes B2 Dimerization. J Virol. 85: 9543-9554.

45. Qiu Y, Cai D, Qi N, Wang Z, Zhou X, Zhang J and Hu Y (2011) Internal initiation is responsible for synthesis of Wuhan nodavirus subgenomic RNA. J Virol, 85: 4440-4451.

46. Zhou X, Si J, Corvera J, Gallick G, Kuang J. Decoding the Intrinsic Mechanism that Prohibits ALIX Interaction with ESCRT and Viral Proteins. (2010) Biochem. J.432(3):525-34.

47. Zhou X,Pan S, Sun L, Corvera J, Lee YC, Lin SH, Kuang J. (2009) The CHMP4b and Src docking sites in the Bro1 domain are autoinhibited in the native state of Alix. Biochem J. 418(2): 277-284.

48. Zhou X, Pan S, Sun L, Corvera J, Lin SH, Kuang J. (2008) The HIV-1 p6/EIAV p9 docking site in Alix is autoinhibited as revealed by a conformation sensitive anti-Alix monoclonal antibody. Biochem J.414(2):215-220.

49. Pan S, Wang R, Zhou X, Kloc M, Corvera J, Koomen J, Kobayashi R, Sifers R, Gallick GE, Lin SH and Kuang J. (2008) Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly. EMBO J.27:2077-2090.

50. Feng Z, Wu CF, Zhou X, Kuang J. Alternative polyadenylation produces two major transcripts of Alix. (2007)Arch Biochem Biophys465:328-335.

51. Pan S, Wang R, Zhou X, He G, Koomen J, Kobayashi R, Sun L, Corvera J, Gallick GE, Kuang J. (2006) Involvement of the conserved adaptor protein Alix in actin cytoskeleton assembly. J Biol Chem281(45):34640-34650.