Title: Principal Investigator E-mail: x.zhang@wh.iov.cn


Xian-En Zhang received his first degree in Hubei University in 1982, MPhil. (microbiology, 1985) and Ph.D. (biochemistry, 1995) in the Chinese Academy of Science (CAS).

Research Interests

1) Nanobiology

we have exploited macrobiomolecules, such as viral capsid proteins and microbial surface layer proteins, as building blocks for construction of nanostructures with designed functions, e.g. biosensing and drug carriers. The current interest focuses on profiling cell surface proteins at super resolution level.

2) Sensing molecular events in living cells

we have developing red, far red and near infrared fluorescent protein molecular sensing systems (BiFC, TriFC, etc.) and "color virus" to achieve the visualization of molecular interactions in living cells and in vivo. The current interests include constructing cell sensors for detection of toxicity of chemicals at subcellular level and building molecular sensors for study of 3D genomics.

3) Biosensors

We have developed a series of biosensor and biochips, including microbial sensors, multiple/cascade enzyme electrodes, gene optode, Mycobacterium tuberculosis proteome microarray for environmental monitoring, bioprocess control, clinical diagnosis and new biomarkers screening. The current study emphasizes portability and flexibility of biosensors.

4) Microbial enzymology for biosensors

we have exploited and engineered a number of microbial enzymes to built some unique methods for improving biosensor performance, e.g. orientation control of enzyme immobilization, homogeneity of biosensor surface, enhanced electron transfer between enzyme molecules and the electrode, cascade enzyme sensors, etc. We continue to promote the research in this area.


1. Minhai Chen, Chuang Yan, Yingxin Ma, Xian-En Zhang*(2020). A tandem near-infrared fluorescence complementation system with enhanced fluorescence for imaging protein-protein interactions in vivo. Biomaterials, 268.  

2. Netrapal Singh, Lin Huang, Dian-Bing Wang, Nan Shao, Xian-En Zhang*(2020). Simultaneous Detection of a Cluster of Differentiation Markers on Leukemia-Derived Exosomes by Multiplex Immuno-Polymerase Chain Reaction via Capillary Electrophoresis Analysis. Analytical Chemistry, 92(15):10569-10577.  

3. Xia Li, Dianbing Wang, Zongqiang Cui, Qin Li, Min Li, Yinxin Ma, Qinxue Hu, Yikai Zhou*, Xian-En Zhang*(2020). HIV-1 viral cores enter the nucleus collectively through the nuclear endocytosis-like pathway. Science China-Life Sciences, DOI: 10.1007/s11427-020-1716-x.  

4. Jin Song, Yiwei Li, Fang Yin, Zhitao Zhang, Dingkun Ke, Dian-bing Wang, Qipeng Yuan*, Xian-En Zhang*(2020). Enhanced Electrochemical Impedance Spectroscopy Analysis of 3Microbial Biofilms on an Electrochemically In Situ Generated Graphene Interface. ACS Sensors, 5(6) 1795-1803.  

5. Song Lu, Dian-Bing Wang, Yu Hou, Dongge Guo, Yulin Deng*, Xian-En Zhang*(2020). Illuminating single genomic loci in live cells by reducing nuclear background fluorescence. Science China-Life Sciences, doi: 10.1007/s11427-020-1794-2.  

6. Chenchen Xu, Weiwei Zhu, Hanjin Mao, Wenjing Zhang, Gen-Quan Yin, Xian-En Zhang*, Feng Li*(2020). Switch from Polymorphic to Homogenous Self-Assembly of Virus-Like Particles of Simian Virus 40 through Double-Cysteine Substitution. SMALL, DOI: 10.1002/smll.202004484.  

7. Wei Feng, Ashley M. Newbigging, Connie Le, Bo Pang, Hanyong Peng, Yiren Cao, Jinjun Wu, Ghulam Abbas, Jin Song, Dian-Bing Wang, Mengmeng Cui, Jeffery Tao, D. Lome, Tyrrell, Xian-En Zhang*, Hongquan Zhang*, X. Chis Le*(2020). Molecular Diagnosis of COVID-19: Challenges and Research Needs. Analytical Chemistry, 15(92),10196-10209.  

8. Xian-En Zhang*. Nanobiology-Symphony of bioscience and nanoscience. Science China-Life Sciences, 8(63), 1099-1102.  

9. Lin Huang, Ning Gu, Xian-En Zhang*, Dian-Bing Wang* (2019). Light-Inducible Exosome-Based Vehicle for Endogenous RNA Loading and Delivery to Leukemia Cells. Advanced Functional Materials, 29(9):1807189.  

10. Yan Xi, Dian-Bing Wang, Tingting Wang, Lin Huang, Xian-En Zhang* (2019). Quantitative Profiling of CD13 on Single Acute Myeloid Leukemia Cells by Super-resolution imaging and its Implication in Targeted Drug Susceptibility Assessment. Nanoscale, 11(4):1737-1744.  

11. Dongge Guo, Dian-Bing Wang, Chong Liu, Song Lu, Yu Hou, Xian-En Zhang* (2019). CRISPR-based genomic loci labeling revealed ordered spatial organization of chromatin in living diploid human cells. BBA-Molecular Cell Research, 1866(12):118518.  

12. Lingling Li, Chengchen Xu, Wenjing Zhang, Francesco Secundo, Chunyan Li, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2019). Cargo-Compatible Encapsulation in Virus-Based Nanoparticles. Nano Letters, 19(4):2700-2706.  

13. Tingting Wang, Guang Li, Dianbing Wang, Feng Li, Dong Men, Tao Hu, Yan Xi, and Xian-En Zhang* (2019). Quantitative profiling of integrin αvβ3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma. Nanoscale, 11(39):18224-18231.  

14. Yiwei Li, Juan Zhou, Jin Song, Xiaosheng Liang, Zhiping Zhang, Dong Men*, Dianbing Wang, Xian-En Zhang* (2019). Chemical Nature of Electrochemical activation of Carbon Electrodes. Biosensors & Bioelectronics, 144: 111534.  

15. Sha Wei, Dianbing Wang, Hua Li, Lijun Bi, Jiaoyu Deng, Guofeng Zhu, Jibin Zhang, Chuanyou Li, Min Li, Yuan Fang, Guimin Zhang, Jian Chen, Shengce Tao, Xian-En Zhang* (2019). Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF‐κB signalling pathway by binding to eEF1A1. Cellular Microbiology, e13090.  

16. Yuanyuan Chen, Zhengyan Zhan, Hongtai Zhang, Lijun Bi, Xian-En Zhang*, Yu Vincent Fu* (2019). Kinetic analysis of DNA compaction by mycobacterial integration host factor at the single-molecule level. Tuberculosis, 119, 101862.  

17. Xian-En Zhang* (2018). Forty years of highly synchronous development in China’s scientific research and economy. National Science Review, 5 (6): 796–798.  

18. Fan Yang, Xiaolei Zuo, Chunhai Fan*, Xian-En Zhang* (2018). Biomacromolecular nanostructures-based interfacial engineering: from precise assembly to precision biosensing. National Science Review, 5 (5): 740–755.  

19. Lin Huang, Dian-Bing Wang, Netrapal Singh, Fang Yang, Ning Gu*, Xian-En Zhang* (2018). A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes. Nanoscale, 10(43): 20289-20295.  

20. Ye Yuan, Dian-Bing Wang, Jibin Zhang, Ji Liu, Jian Chen, Xian-En Zhang* (2018). Raman spectra of the GFP-like fluorescent proteins. Biophysics reports, 4(5): 265-272.  

21. Jigang Wang, Ti Fang, Ming Li, Wenjing Zhang, Zhi-Ping Zhang, Xian-En Zhang*, Feng Li* (2018). Intracellular delivery of peptide drugs using viral nanoparticles of bacteriophage P

22: covalent loading and cleavable release. Journal of Materials Chemistry B, 6(22):3716-3726.  

23. Wen Yin, Wei Li, Qin Li, Chong Qin, Zhi-Ping Zhang, Xiaowei Zhang, Xian-En Zhang*, Zongqiang Cui* (2018). Structural Characterization and Analysis of Human Immunodeficiency Virus Encapsulating Quantum Dots. Journal of Nanoscience and Nanotechnology, 18(7):4487-4494.  

24. Wenjing Zhang, Xian-En Zhang*, Feng Li* (2018).Virus-Based Nanoparticles of Simian Virus 40 in the Field of Nanobiotechnology. Biotechnology Journal, 13(6).  

25. Dong Men, Juan Zhou, Wei Li, Cui-Hua Wei, Yuan-yuan Chen, Kun Zhou, Ying Zheng, Ke Xu, Zhi-Ping Zhang, Xian-En Zhang* (2018). Self-Assembly of Antigen Proteins into Nanowires Greatly Enhances the Binding Affinity for High-Efficiency Target Capture. ACS Appl Mater Interfaces, 10(48):41019-41025.  

26. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Guoqiang Wu, Tianwei Tan, Zongqiang Cui*, Xian-En Zhang* (2017). Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System. Analytical Chemistry, 89(23): 12896-12901.  

27. Qin Li, Wei Li, Wen Yin, Jia Guo, Zhi-Ping Zhang, Dejun Zeng, Xiaowei Zhang, Yuntao Wu, Xian-En Zhang*, and Zongqiang Cui* (2017). Single-Particle Tracking of Human Immunodeficiency Virus Type 1 Productive Entry into Human Primary Macrophages. Acs Nano, 11(4):3890-3903.  

28. Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang* & Zongqiang Cui* (2017). Live cell imaging of single genomic loci with quantum dot-labeled TALEs. Nature Communications, 8:15318.  

29. Kun Li, Ting Li, Shan-shan Yang, Xu-De Wang, Lei-xin Gao, Rui-Qi Wang, JingGu, Xian-En Zhang*, Jiao-Yu Deng* (2017). Deletion of nudB causes increased susceptibility to antifolates in Escherichia coli and Salmonella enterica. Antimicrobial Agents & Chemotherapy, 61(5):02378-16.  

30. Hua Li, Sha Wei, Yuan Fang, Min Li, Xia Li, Zhe Li, Jibin Zhang, Guofeng Zhu, Chuanyou Li, Lijun Bi, Guimin Zhang, Dianbing Wang,* and Xian-En Zhang* (2017).Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells. ABBS, 49 (9):835-844.  

31. Zhitao Zhang, Dianbing Wang, Chuanyou Li, Jiaoyu Deng, Jibin Zhang, Lijun Bi and Xian-En Zhang* (2017). Microbial sensor for drug susceptibility testing of Mycobacterium tuberculosis. J Appl. Microbiol, 124(1): 286-293.  

32. Jing Gu, Yuanyuan Chen, Hongsen Guo, Manluan Sun, Mingkun Yang, Xude Wang, Xian-En Zhang*, and Jiaoyu Deng* (2017). Lysine acetylation regulates the activity of Escherichia coil pyridoxine 5'-phosphate oxidase. ABBS, 49(2):186-192.  

33. Xianxun Sun, Wei Li, Xiaowei Zhang, Mi Qi, Zhiping Zhang, Xian-En Zhang*, Zongqiang Cui* (2016). In Vivo Targeting and Imaging of Atherosclerosis Using Multifunctional Virus-Like Particles of Simian Virus 40. Nano Letters, 16:6164-6171.  

34. Yingxin Ma, Zhike He, Tianwei Tan, Wei Li, Zhiping Zhang, Shuang Song, Xiaowei Zhang, Qinxue Hu, Peng Zhou, Yuntao Wu, Xian-En Zhang* and Zongqiang Cui* (2016). Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles. ACS Nano, 10: 6273-6282  

35. Minghai Chen, Sanying Liu, Wei Li, Zhiping Zhang, Xiaowei Zhang, Xian-En Zhang*, Zongqiang Cui* (2016). Three-Fragment Fluorescence Complementation Coupled with Photoactivated Localization Microscopy for Nanoscale Imaging of Ternary Complexes. ACS Nano, 10: 8482-8490.  

36. Dong Men*, Juan Zhou, Wei Li, Yan Leng, Xinwen Chen, Sheng-ce Tao, Xian-En Zhang* (2016). Fluorescent Protein Nanowire-Mediated Protein Microarrays for Multiplexed and Highly Sensitive Pathogen Detection. ACS Applied Matrrials & Interfaces, 8:17472-17477.  

37. Xian-En Zhang*, Zongqiang Cui, Dianbing Wang (2016). Sensing of biomolecular interactions using fluorescence complementing systems in living cells. Biosensors & Bioelectronics, 76:243-250.  

38. Yingxin Ma, Zongqiang Cui, Zhike He, Wei Li, Zhi-Ping, Zhang, Xiaowei Zhang, Xian-En Zhang*, Tianwei Tan* (2016). Delaying Photobleaching of a Light-Switch Complex for Real-Time Imaging of Single Viral Particle Uncoating. Analytical Chemistry, 88:10675-10679.  

39. Ting Wang, Zhenhua Zheng, Xian-En Zhang*, Hanzhong Wang* (2016). Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions. Talanta, (2016)179–184.  

40. ZhaoYang Li, ZhiPing Zhang, LiJun Bi, ZongQiang Cui, JiaoYu Deng, DianBing Wang*, Xian-En Zhang* (2016). Mutagenesis of mNeptune Red-Shifts Emission Spectrum to 681-685 nm. PLOS ONE, 11(4):e0148749.