Title: Principal Investigator E-mail:


1998, B.S. Chemistry, Tsinghua University, Beijing, China

2001, B.S. Chemistry, Tsinghua University, Beijing, China

2006, Ph.D. Chemistry, University of Massachusetts Amherst, Amherst, MA, USA

Research Interests

The RNA-dependent RNA polymerases (RdRPs) play essential roles in RNA virus genome replication. The research in my laboratory focuses on the mechanisms of catalysis and regulation of viral RdRPs as well as the development of RdRP-based antiviral strategies.



1.  Yang J, Jing X, Yi W, Li XD, Yao C, Zhang B, Zheng Z, Wang H, Gong P. 2021. Crystal structure of a tick-borne flavivirus RNA-dependent RNA polymerase suggests a host adaptation hotspot in RNA viruses. Nucleic Acids Res 49:1567-1580.

2.    Wang Q, Wu J, Wang H, Gao Y, Liu Q, Mu A, Ji W, Yan L, Zhu Y, Zhu C, Fang X, Yang X, Huang Y, Gao H, Liu F, Ge J, Sun Q, Yang X, Xu W, Liu Z, Yang H, Lou Z, Jiang B, Guddat LW, Gong P, Rao Z. 2020. Structural Basis for RNA Replication by the SARS-CoV-2 Polymerase. Cell doi:10.1016/j.cell.2020.05.034.

3.  Wang M, Li R, Shu B, Jing X, Ye HQ, Gong P. 2020. Stringent control of the RNA-dependent RNA polymerase translocation revealed by multiple intermediate structures. Nat Commun 11:2605.

4.  Wu J, Ye HQ, Zhang QY, Lu G, Zhang B, Gong P. 2020. A conformation-based intra-molecular initiation factor identified in the flavivirus RNA-dependent RNA polymerase. PLoS Pathog 16:e1008484.

5.  Shi W, Ye HQ, Deng CL, Li R, Zhang B, Gong P. 2020. A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability. Nucleic Acids Res 48:1392-1405.

6.  Jia H, Gong P. 2019. A Structure-Function Diversity Survey of the RNA-Dependent RNA Polymerases From the Positive-Strand RNA Viruses. Front Microbiol 10:1945.

7.  Fang J, Jing X, Lu G, Xu Y, Gong P. 2019. Crystallographic Snapshots of the Zika Virus NS3 Helicase Help Visualize the Reactant Water Replenishment. ACS Infect Dis 5:177-183.

8.  Jing X, Yuan Y, Wu Y, Wu D, Gong P, Gao M. 2019. Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis. Protein Sci 28:609-619.

9.  Wu J, Gong P. 2018. Visualizing the Nucleotide Addition Cycle of Viral RNA-Dependent RNA Polymerase. Viruses 10.

10.Liu W, Shi X, Gong P. 2018. A unique intra-molecular fidelity-modulating mechanism identified in a viral RNA-dependent RNA polymerase. Nucleic Acids Res doi:10.1093/nar/gky848.

11.Lu G, Gong P. 2017. A structural view of the RNA-dependent RNA polymerases from the Flavivirus genus. Virus Res 234:34-43.

12.Shu B, Gong P. 2017. The uncoupling of catalysis and translocation in the viral RNA-dependent RNA polymerase. RNA Biol 14:1314-1319.

13.Shi W, Gong P. 2017. A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase. Virol Sin 32:331-334.

14.Zheng F, Lu G, Li L, Gong P, Pan Z. 2017. Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3. J Virol 91.

15.Kuang W, Zhang H, Wang M, Zhou NY, Deng F, Wang H, Gong P, Hu Z. 2017. Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function. J Virol 91.

16.Bi P, Shu B, Gong P. 2017. Crystal structure of the coxsackievirus A16 RNA-dependent RNA polymerase elongation complex reveals novel features in motif A dynamics. Virol Sin 32:548-552.

17.Lu G, Gong P. 2016. Natural polymerase fusion as an initiation regulator? Oncotarget 7:35498-35499.

18.Shu B, Gong P. 2016. Structural basis of viral RNA-dependent RNA polymerase catalysis and translocation. Proc Natl Acad Sci U S A 113:E4005-14.

19.Wu J, Liu W, Gong P. 2015. A Structural Overview of RNA-Dependent RNA Polymerases from the Flaviviridae Family. Int J Mol Sci 16:12943-57.

20.Wu J, Lu G, Zhang B, Gong P. 2015. Perturbation in the conserved methyltransferase-polymerase interface of flavivirus NS5 differentially affects polymerase initiation and elongation. J Virol 89:249-61.

21.Chen L, Yan Q, Lu G, Hu Z, Zhang G, Zhang S, Ding B, Jiang Y, Zhong Y, Gong P, Chen M. 2015. Several residues within the N-terminal arm of vesicular stomatitis virus nucleoprotein play a critical role in protecting viral RNA from nuclease digestion. Virology 478:9-17.

22.Lu G, Gong P. 2013. Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface. PLoS Pathog 9:e1003549.

23.Gong P, Peersen OB. 2010. Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase. Proc Natl Acad Sci U S A 107:22505-10.

24.Gong P, Kortus MG, Nix JC, Davis RE, Peersen OB. 2013. Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts. PLoS One 8:e60272.