Yang H, Wang M, Yu J, Wei H.
FEMS Microbiol Lett. 2015 Feb 16. pii: fnv025.
Abstract
Biofilm formation renders Staphylococcus aureus highly resistant to conventional antibiotics and host defenses. Four D-amino acids (D-Leu, D-Met, D-Trp and D-Tyr) have been reported able to inhibit biofilm formation and disassemble established S. aureus biofilms. We reported here for the first time that both D- and L-isoforms of aspartate (Asp) inhibited S. aureus biofilm formation on tissue culture plates. Similar biofilm inhibition effects were also observed against other staphylococcal strains, including S. saprophyticus, S. equorum, S. chromogenes, and S. haemolyticus. It was found that Asp at high concentrations (above 10 mM) inhibited the growth of planktonic N315 cells, but at sub-inhibitory concentrations decreased the cellular metabolic activity without influencing the cell growth. The decreased cellular metabolic activity might be the reason for producing less proteins and DNA in the matrix of the biofilms formed in the presence of Asp. However, varied inhibition efficacies of Asp were observed for biofilms formed by clinical staphylococcal isolates. There might be mechanisms other than decreasing the metabolic activity, e.g., the biofilm phenotypes, affecting the biofilm formation in the presence of Asp.
http://www.ncbi.nlm.nih.gov/pubmed/25687923
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