Interactions among Dendrolimus punctatus cypovirus proteins and identification of the genomic encoding of its A-spike

Abstract

　　Revealing the interactions among cypovirus (CPV) proteins would facilitate understanding of viral replication and assembly. In the present study, interactions among proteins encoded by the 10 segments of Dendrolimus punctatus cypovirus (DpCPV) were identified using yeast two-hybrid (Y2H) and far-western blotting assays. In total, 24 pairs of interactions were detected. Twelve pairs of one-direction interactions, four pairs of binary interactions, and four pairs of self-associations were identified in the Y2H assays. Another four pairs of interactions were identified by far-western blotting. The interactions between the methyltransferase domain of turret protein (VP3) and VP4 as well as between polyhedrin and VP4 were further confirmed by far-western blotting and pull-down assays, respectively. In addition, the immuno-gold labeling showed that the A-spike of DpCPV was formed by VP4. In conclusion, we obtained the protein-protein interaction network of DpCPV and identified that its A-spike was formed by VP4 encoded by genomic segment 6.

http://vir.sgmjournals.org/content/early/2014/04/03/vir.0.064022-0.abstract