A cis-encoded sRNA controls the expression of fabH2 in Yersinia

Pei Lu,Yong Zhang, Yangbo Hu, Matthew S.Francis, Shiyun Chen

FEBS Letters.Volume 588, Issue 10, 21 May 2014, Pages 1961–1966.DOI: 10.1016/j.febslet.2014.04.005

Abstract

　　YsrH is a novel cis-encoded sRNA located on the opposite strand to fabH2, which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH-mediated regulation of fabH2 expression was investigated in Yersinia pseudotuberculosis. Constitutive and inducible over-expression of YsrH decreased the mRNA level of fabH2, while expression of downstream fabD and fabG remained unaffected. Polynucleotide phosphorylase (PNPase) also played an important role in this regulation process by mediating YsrH decay in the exponential phase. Thus, our data defines a cis-encoded sRNA that regulates fatty acid synthesis via a regulatory mechanism also involving PNPase.

http://www.sciencedirect.com/science/article/pii/S0014579314002816