Wu D, Yuan Y, Liu P, Wu Y, Gao M.Cellular responses in Bacillus thuringiensis CS33 during bacteriophage BtCS33 infection. J Proteomics. 2014 Feb 22. pii: S1874-3919(14)00065-7. doi: 10.1016/j.jprot.2014.02.016. [Epub ahead of print]
Abstract Bacillus thuringiensis (Bt) has been widely used for 50years as a biopesticide for controlling insect pests However, bacteriophage infection can cause failures in 50%-80% of the batches during Bt fermentation, resulting in severe losses. In the present work, the physiological and biochemical impacts of Bt strain CS33 have been studied during bacteriophage infection. This study adopted a gel-based proteomics approach to probe the sequential changed proteins in phage-infected Bt cells. To phage, it depressed the host energy metabolism by suppressing the respiration chain, the TCA cycle, and the utilization of PHB on one hand; on the other hand, it hijacked the host translational machine for its own macromolecular synthesis. To host, superinfection exclusion might be triggered by the changes of S-layer protein and flagella related proteins, which were located on the cell surface and might play as the candidates for the phage recognition. More importantly, the growth rate, cell mass, and ICPs yield were significantly decreased. The low yield of ICPs was mainly due to the suppressed utilization of PHB granules. Further functional study on these altered proteins may lead to a better understanding of the pathogenic mechanisms and the identification of new targets for phage control.
http://www.ncbi.nlm.nih.gov/pubmed/24565692
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